Microbial Systematics : PYQ

 Chapter 1: Bacterial Systematics

-Q. Write a note on 5 kingdom classification.

Q. Discuss in breif diversity of seconadary metabolites.

--Q. Discuss in brief 3 domain classification system.

--Q. What is species in eukaryotes?

Q. Explain neo-darwinism

--Q. Species concept of prokaryotes.

-Q. Comment on pehentic approach in determinative bacteriology.

--Q. Write note on phylogenic approach in systematic bacteriology.

Q. Explain phylogenetic tree. Explain how it is constructed.

-Q. What is phylognetic tree ? How it is constructed?

--Q. Write note on polyphasic approach in bacterial taxonomy.

Q. Describe and explain the phenetic approach of classification with suitable example.

Chapter 2: Microbial Diversity

Q. Write a note on measurement of microbial diversity.

-Q. Briefly explain the expanse of microbial diiversity.

-Q. Write a note on estimates of total number of microbial species.

Q. Explain species divergence. Enlist the techniques for measurement of microbial diversity.

Q. Jsutify: 16S rRNA is most widely accepted moelcular chronometer in bacterial taxonomy.

Chapter 3: Measurement of unculturable microbial diversity

--Q. Justify : “Morphological characterization is adequate for fungal classification upto class level”.

Q. Justify: Classification of molds is chiefly based on their morphological characters.

Q. Write a note on applications of FISH in bacterial taxonomy.

-Q. Explain various approaches to access the total number of bacteria in environment.

-Q. Explain the strategies used for culturing unculturable bacteria.

Q. Enlist different types of bacterial diversity indices.Discuss any one with suitable example.

---Q. Give the protocol for extracting total bacterial DNA from habitat.

-Q. Culture independent molecular methods for identifying unculturable bacteria, explain any one.

--Q. Explain different molecules used as molecular clocks in bacterial taxonomoy.

Q. What are universal primers? Explain how these are applied in microbial taxnomoy and diversiity.

Q. Proteri profiling in bacterial taxonomy.

Chapter 4: Evolution

---Q. Write a note on neutral and co-evolution.

Q. Emlist levels of selection

---Q. Discuss the concept of evolutionary r and k selection.

-Q. Elaborate on the concept of ‘unculturable’ bacterial diversity.

Q. Write note on strategies for culture of ‘unculturable’ bacteria.

Q. Explain concept of neutral evolution with example.

-Q. Explain evolution with respect to secondary metabolites.

-Q. Define molecular evolution.

Give the flowsheet diagram for etimating total number of specie from given environment

Enlist the different classes of fungi with examples.

Give principle of PAGE and add a note on its applications

Give significance of stability of diversity. 

Write note on metagenome analysis.

---Q. Salient features of zygomycetes.

---Describe the salient features of Basidiomycetes. 

--Differentiate basidiomycetes and ascomycetes.

Give outline of classification of fungi.

Justify : “Gel concentration regulates the mobility of test molecule during electrophoresis”.

Write note on whole genome shotgun sequencing.

Explain challenges in gene sequencing.

Explain great plate cont anamolies

shannon diversity