Repeated questions

1: Seperation and analysis of biomolecules
Q. What is FPLC.
--Q. a) The components of Gas liquid chromatography.
Q. Enlist detectors for gas chromatography.
Q. Enlidt detectors for HPLC.
b) The working of Analytical ultra centrifuge.
Q. Principle an dworking of HPLC
-Q. Explain principle and working of gel electrophoresis.
Q. What is isoelectric focusingg.

2: Spectroscopy


-c) Explain the working of MALDI-TOF.
c) The working of Fluorescent spectrometer.
Q. Draw and explain compoenents of Fluerescnece spectroscopy.
c) Explain Ionization of molecules in Mass spectro photometry.
---Q. What is infrared spectroscopy 
Q. Describe componenets of ir spectrosope.
-Q. What is FTIR.
Q. Waht are analyzers used in masss chromatography.
--Q. Components of mass spectrometer.
Q. Give principle of GCMS.
Q. Describe in brief mass spectroscopy,
Q. Give instrumentation for x ray crystallography.
=Q. Describe siingle crystal x ray crystallography.
-Q. Crystallization of proteins for X ray crystallography,
- Q. What is beer lambert's law? What is its role in study of macromolecules using UV-VIS setrophotometer.
Q. What is FRET.
Q. What is bathochromic and hypochromic shift.
Q. Write a short note on ion exchange chromatography.

3: Biophysical Techniques

Q. Describe super secondary structures of protein.
Q.With suitable example explain quarternary structure of protien.
-a) How is phase determination done in X-ray crystallography.
-Q. Explain principle of x ray diffraction.


b) With respect to NMR explain
i) Relaxation parameter
--ii) Spin-spin coupling
---iii) chemical shift
Q. a) Homology based Method for protein structure prediction.
c) With a suitable example explain tertiary structure of Protein.
a) Explain basic principle of X ray diffraction.
-b) Explain basic principle of NMR and the working of NMR instrument.
-q. Applications of NMR.
-Q. Differentiate between reciprocal and direct lattice.



4: Radioisotopes in Biology and Confocal Microscopy
--a) Explain measurement of Radio activity using Scintillation counters.
Q. Explain liquid scintillation counting.
Q. What are radioisotopes and describe their characteristics.
Q. Give priciple of cofocal microscopy


Q3) Attempt any two of the following : [16]


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