2022 que papers sem 2

 Nitrogen metabolism respiration and photosynthesis

Q1) Attempt any five of the following. [5]

 a) Write down net reaction of biological N2 reduction.

 b) Enlist component involved in bacterial cyclic electron flow of photosynthesis.

 c) Name the substrates used for the reactions carried out by Rubisco.

 d) If radioactively labelled water H2 18O is provided to plant, where does the label appear.

 e) What is IMP? What are the products formed from IMP.

 f) What is full form of PRPP?

 Q2) Attempt the following.

 a) Describe photophosphorylation in purple Sulphur bacteria. Compare it with photosystem II in plants. [6] 

b) Enlist the properties of nitrogenase enzyme. [4] 

 Q3) Attempt the following. 

a) Explain CAM pathway and give its significance. [6] 

b) Write short note on Oxygen Evolving Complex (OEC). [4] 

Q4) Attempt the following. 

a) Differentiate between C3 and C4 plants. [6] 

b) Justify, "enzymes involved in nitrogen metabolism like glutamine synthetase and nitrogenase can be regulated by covalent modification". [4]

 Q5) Attempt any two of following. [10] 

a) What is photorespiration? What is its cause and why is it believed to be wasteful? 

b) Describe biosynthesis of pyruvate family of amino acids.

c) Write note on regulation of Calvin cycle. 

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MBCT - 121 : INSTRUMENTATION AND MOLECULAR BIOPHYSICS

Q1) Attempt any five : [10] a) Explain column efficiency. b) Enlist detectors far HPLC. c) Define chemical shift in NMR spectroscopy. d) What is bravis lattice? e) What are radioactive isotopes? f) What is quenching? Q2) Attempt the following: a) Explain instrumentation, working & application of HPLC. [7] b) Explain partition coefficient and resolution of column chromatography. [5] Total No. of Questions : 7] [Total No. of Pages : 2 [5913]-21 M.Sc. (Microbiology) MBCT - 121 : INSTRUMENTATION AND MOLECULAR BIOPHYSICS (2019 Pattern) (Semester - II) (Credit System) (Revised) Time : 3 Hours] [Max. Marks : 70 Instructions to the candidates: 1) Q.1 is compulsory. 2) Solve any five questions from Q.2 to Q.7. 3) Q.2 to Q.7 carry equal marks. 4) Figures to the right side indicate full marks 5) Draw neat labelled diagrams wherever necessary. 6) Use of scientific calculator is allowed. 7) Assume suitable data, if necessary. PA-3270 SEAT No. : P.T.O. [5913]-21 2 Q3) Attempt the following: a) Describe different mass analysers and ionization of molecules in mass spectrometry. [7] b) The absorbance A of 5 × 10–4 M solution of amino acid tyrosine at wavelength of 280nm is 0.75. The path length of the cuvette is 1 cm. What is molar absorption coefficient ε? [5] Q4) Attempt the following: a) Explain the basis principle of NMR and working of NMR instrument with suitable diagram. [7] b) Explain direct lattice & reciprocal lattice. [5] Q5) Attempt the following: a) Explain measurement of radioactivity using scintillation counters. [7] b) With a suitable example explain the pluse chase experiment. [5] Q6) Attempt the following: a) Explain working of MALDI - TOF. [7] b) Describe the principle of isoelectric focusing. [5] Q7) Write short note on any Two of the following: [12] a) SDS - PAGE. b) Signal to noise ratio. c) Eward sphere.

MBCT - 122 (MB - 602) : MOLECULAR BIOLOGY

Q1) Attempt any five : [10] a) Comment on use of T4 DNA polymerase. b) Write applications of genome project. c) Give applications of knock out mice. d) Explain construction of Ti based vector. e) What is spliceosome? f) What is gene annotation? Q2) a) Enlist various methods used for designing probes and explain any two of them in detail. [7] b) Explain about the vectors derived from pichia and its applications.[5] Q3) a) Justify : 'RNA interference technique can be used in gene silencing:[7] b) Explain the use of micro RNA in cancer diagnosis. [5] Total No. of Questions : 7] [Total No. of Pages : 2 [5913]-22 M.Sc. (Microbiology) MBCT - 122 (MB - 602) : MOLECULAR BIOLOGY (2019 Pattern) (Semester - II) (CBCS) Time : 3 Hours] [Max. Marks : 70 Instructions to the candidates: 1) Q.1 is compulsory. 2) Solve any five questions from Q.2 to Q.7. 3) Q.2 to Q.7 carry equal marks. 4) Draw neat labelled diagrams wherever necessary. 5) Figures to the right indicate full marks 6) Assume suitable data, if necessary. 7) Use of scientific calculator is allowed. PA-3271 SEAT No. : P.T.O. [5913]-22 2 Q4) a) What are the salient features of human genome project. [7] b) What are expression vectors? Explain use of expression vectors with suitable examples. [5] Q5) a) Comment on use of immunoassay. [7] b) As a part of under graduate project, a student was attempting to construct a restriction map of the plasmid pVC 23 using restriction enzymes Eco RI and Bam HI, after carrying out both single and double enzyme digest reactions, following fragments were obtained. [5] Enzyme(s) Fragment length obtained Eco RI ................................... 20 kb Bam HI ................................ 11 kb, 6 kb, 3 kb Eco RI + Bam HI ................ 8 kb, 6 kb, 3 kb(2) From the information construct a restriction map of pVC 23. Q6) a) Write a note on restriction endonucleases and their applications in molecular biology. [7] b) Describe RNA splicing. [5] Q7) Attempt the following: [Any 2] [12] a) Give protocol for preparing gene library. b) Write a note on - genome project of drosophila. c) Comment on : RNA signatures of antibiotic resistance.

MBCT 123 : Enzymology, Bioenergetics and Metabolism

Q1) Attempt any Five : [10] a) What are sugar epimers? b) Enlist different methods used for purification of an enzyme. c) Define Gibb’s free energy. d) Define Kcat. e) Write down two examples of saturated fatty acids with structures. f) State third law of thermodynamics. Q2) Attempt the following : a) Describe in detail the role of TCA cycle in generating biosynthetic intermediates. [7] b) Explain with the help of suitable example the construction of an enzyme purification chart. [5] Total No. of Questions : 7] [Total No. of Pages : 3 [5913]-23 M.Sc. - I MICROBIOLOGY MBCT 123 : Enzymology, Bioenergetics and Metabolism (2019 Pattern) (CBCS) (Semester - II) Time : 3 Hours] [Max. Marks : 70 Instructions to the candidates: 1) Q. 1 is compulsory. 2) Solve any five questions from Q.2 to Q.7. 3) Q. 2 to Q. 7 carry equal marks. 4) Figures to the right side indicate full marks. 5) Draw neat labelled diagrams wherever necessary. 6) Use of scientific calculator is allowed. 7) Assume suitable data if necessary. PA-3272 SEAT No. : P.T.O. [5913]-23 2 Q3) Attempt the following : a) Explain concept of an allosteric enzymes with suitable example. [7] b) Calculate G for hydrolysis of ATP at pH7 & 25°C under steady state conditions (such as might exist in a living cell) in which the concentrations of ATP, ADP & Pi are maintained at 10–5M, 10–4M & 10–3M respectively. [5] (Given G10 = –7700 call mole) Q4) Attempt the following : a) What are coupled reactions? Discuss the significance of high energy compounds in such reactions. [7] b) Draw secondary plots for uncompetitive inhibition & comment on calculation of Ki. [5] Q5) Attempt the following : a) Phosphoglucomutase is crucial for glycogen breakdown as well as glycogen synthesis. Explain the role of this enzyme in each of the two processes. [7] b) A marine microorganism contains an enzymes that hydrolyzes glucose 6 sulfate. The assay is based on rate of glucose formation. The enzyme is a cell free extract has kinetic constants of Km = 6.7 × 10–4M & Vmax = 300nmols/lit–1/min–1. Galactose 6 PO4 is a competitive inhibitor (I). At 10–5 galactose-6-sulphate & 2 × 10–5M glucose-6- sulfate,  was 1.5nmole × lit–1/min–1. Calculate Ki for galactose-6- sulfate. [5] Q6) Attempt the following : a) Comment on entropy. [7] b) What relationship exist in Km & S in enzyme catalysed reaction proceeds at 75% Vmax. [5] [5913]-23 3 Q7) Attempt any two of the following : [12] a) Write short note on phospholipids. b) Outline pathway of biosynthesis of sterols. c) Write short note on Atkinson’s energy charge.