2019

 MB - 601 : Instrumentation & Molecular Biophysics

Q1) Attempt any two of the following. [10] a) Explain significance of van deemter equation in chromatography. b) Explain any two applications of affinity chromatography. c) A macromolecule with V partial specific volume is 0.74 cm3/g is redimented in water at 20ºC S is 14.210–13 , D is 5.8210–6 , R is 8.3100107 calculate its mole cular weight. Q2) Attempt any two of the following. [10] a) Enlist different methods of ionization in Mass Spectroscopy and explain any one method in detail. b) Diagrammatically represent circular dichroism Instrument and Explain any 2 applications of C.D. c) Write short note on : FRET (Fluorescence Resonance Energy Transfer) Q3) Attempt any two of the following. [10] a) Explain Direct Lattice and Reciprocal Lattice seen in x-ray crystallography b) Explain Chemical Shift and Intensity with respect to NMR c) Comment on : NOESY P.T.O. 2 [5534]-201 Q4) Attempt any two of the following. [10] a) Explain electron density map obtained in x-ray crystallography. b) Justify : Isoelectric foccusing is used in purification of amphoteric molecules like proteins c) Enlist detectors used in HPLC and Explain the working of any two detectors. Q5) Attempt any two of the following. [10] a) With suitable examle explain tertiary structure of protein. b) Explain secondary structures of proteins. c) Write short note on : Ramchandran plot. Q6) Attempt any two of the following. [10] a) Comment on : OMIM database b) Explain steps involved in BLAST c) Explain Homology modelling for protein structure prediction. Q7) Attempt any two of the following. [10] a) Explain characteristics of Nano particles b) What are Biogenic Nano particles? Explain synthesis of Nanoparticles by using any one micro organism. c) Explain the significance of SEM in Nanobiotechnology Q8) Attempt any two of the following. [10] a) Explain partial double bond nature of peptide bond. b) Explain pair wise sequence alignment c) Explain Zeta Analysis to Characterize Nanoparticles.