april 2022 sem 2 que papers

 MB - 802 : Molecular Biology - II

Q1) Attempt any two of the following: [10] a) Explain the principle of pyrosefuencing method. b) Elaborate gene imprinting with suitable example. c) Explain the role of geromic variation in trade offs. Q2) Attempt any two of the following: [10] a) Give structural details of YAC and Enlist uses of YAC in cloning. b) Enlist methods for gene transfer to host cell. Explain any one in detail. c) Give the protocol of preparing genomic library. Q3) Attempt any two of the following: [10] a) How will you prepare ‘gum’ using RDT. b) How will you produce novel antibiotics using RDT. c) Explain the production of tryptophan using genetically modified microbes. Q4) Attempt any two of the following: [10] a) What is the correlation between SNP and certain diseases? b) Explain protein engineering with suitable example. c) Write a note on importance of production of high quality protein drug by unconventional microbial system. P.T.O. [5834] - 11 2 Q5) Attempt any two of the following: [10] a) State the applications of GEMO in medicine. b) Discuss why transgenic plants are better than their wild varieties. c) Give advantages of transgenic animals. Q6) Attempt any two of the following: [10] a) Enlist various agricultural wastes that supply starch for the production of fructase. Explain use of genetically modified bacteria in the degradation of starch. b) Give a stepwise protocol in the degradation of xenobiotic compounds. c) Comment on silage production. Q7) Attempt any two of the following: [10] a) State salient features of HGP. How are they different from mouse genome project. b) Give protocol of gene annotation. c) What are the findings of rice genome project? Q8) Attempt any two of the following: [10] a) Comment on gene augmentation. b) State the role of genetically modified Pseudomonas species in the degradation of Xenobiotics. c) What are the applications of HGP? 

MBCT-121: Instrumentation and Molecular Biophysics

Q1) Attempt any five. [10] a) Enlist detectors for Gas chromatography. b) Give two applications of infra red spectroscopy. c) Define chemical shift in NMR spectroscopy. d) What is the source and wavelength range of x-rays? e) Give principle of confocal microscope. f) What is FTIR? Q2) Attempt the following. a) Describe the construction and working of Gas chromatography comment on different types of detectors used in gas chromatography. [7] b) Describe pulse field gel electrophoresis in detail. [5] Q3) Attemp the following: a) Explain principle, instrumentation and applications of infrared spectroscopy. [7] b) If solution containing ATP is found to have absorbanle of 0.17 in 1cm cuvette and the molar extinction coefficient is 1.54×104 cmol dm–3 k-1 m-1 what is the concentration and transmission of ATP solution? [5] P.T.O. [5834] - 201 2 Q4) Attempt the following. a) Explain chemical shift and spin spin coupling with respect to NMR. [7] b) Give an overall approach to structure determination by 2-D NMR. [5] Q5) Attempt the following. a) Explain measurement of Radioactivity using scintillation counters. [7] b) Explain Bathochromic shift and Hypochromic shift in detail. [5] Q6) Attempt the following. a) Give the schematic diagrammatical representation of single beam and double beam UV-Visible spectrophotometer. [7] b) Give applications of Radio Tracer technique in biology. [5] Q7) Write short notes on any Two of the following. [12] a) FRET b) Miller indices c) Fast protein liquid chromatography. 

MBCT - 122: Molecular Biology

Q1) Attempt any five. [10] a) Give function of alkaline phosphatase. b) Write about preparation of knock out mice. c) What is FISH? Give its applications. d) Define RNA interferance. e) Differentiate between mi RNA and si RNA. f) What is RNA splicing. Q2) a) Explain how bacteriophages can be used as vectors. [7] b) Comment on : Activity gel assay. [5] Q3) a) What are expression vectors? Explain use of expression vectors with suitable examples. [7] b) Describe how protein micro array system works. [5] Q4) a) Describe techniques used in deciphering genome. [7] b) Explain construction of cDNA library. [5] P.T.O. [5834] - 202 2 Q5) a) Justify: Western blotting helps in detection of protein of interest. [7] b) A pre mRNA molecule produced from a coding gene has 300 bases long exon I followed by 150 bases intron I, 40 bases exon II, 100 bases intron II, 60 bases exon III and 200 bases adenylated tail. i) Draw the diagram of pre mRNA molecule. ii) Determine the size of full mature mRNA. [5] Q6) a) Explain disease associated changes in gene expression using micro array. [7] b) Explain use of adapters and linkers in genetic enegineering. [5] Q7) Attempt any two of the following. [12] a) Explain use of DNA ligase and T4 DNA polymerase as tools in genetic engineering. b) Write a note on comparative genomics. c) Write a note on protein tagging & purification. 

MBCT - 123: Enzymology, Bioenergetics & Metabolism 

Q1) Attempt any Five. [10] a) What are unsaturated fatty acids? b) Define enthalpy. c) State 2nd law of thermodynamics. d) Give two examples of sugar acids. e) Define allosteric enzymes. f) What are sugar anomers? Q2) Attempt the following. a) Describe in detail synthesis of fatty acids. [7] b) Draw secondary plots for non competitive inhibition. [5] Q3) Attempt the following. a) What are allosteric enzymes? Compare & contrast between KNF & MWC model. Derive kinetic equation for MWC model. [7] P.T.O. [5834] - 203 2 b) Calculate G' for complete oxidation of lactic acid to CO2 & H2 O given the information below. How many moles of ATP could be synthesized in the process at 40% efficiency? [5] Given i) Glucose  2 lactic acid G1   = – 52000 cal / mole ii) Glucose + 6O2  6CO2 +6H2 O G2   = – 686,000 cal / mole Q4) Attempt the following. a) Discuss steps involved in king Altman approach to derive any two substrate enzyme catalysed reaction. [7] b) Explain the steps involved in glucogeogenesis with structure. [5] Q5) Attempt the following. a) Discuss in detail steps involved in beta oxidation process. [7] b) Calculate G°' values for the reaction given below FADH2 + 2 Cytochrome 3 C Fe FAD 2H 2e       (Given 1 E 0.18V 0   & 1 E 0.25V 0   F = 23, 063) [5] Q6) Attempt the following. a) Explain construction of purification chart with suitable example. [7] b) Write a note on Atkinson’s energy charge. [5] Q7) Attempt any two of the following. [12] a) Short note on nomen clature of fatty acids. b) Short note on high energy compounds. c) Short note on Hill plot.

MBET - 127:Nitrogen Metabolism, Respiration and Photosynthesis

Q1) Attempt any five of the following. [5] a) Write down overall stoichiometry of light reactions. b) Write down reaction carried by glutamine synthase. c) Write balanced equation of calvin cycle. d) What is full form of Rubisco? e) What is nitrate respiration? f) Name the precursor molecule used for Histidine biosynthesis. Q2) Attempt the following. a) Describe biochemistry of methanogenesis. [6] b) Write short note on glutamate synthase. [4] Q3) Attempt the following. a) Give the pathway leading to the synthesis of pyrimidine nucleus. [6] b) Explain the cycle flow of electrons in plant photosynthesis. [4] P.T.O. [5834] - 206 2 Q4) Attempt the following. a) Justify, “most of the pathway of amino acid biosynthesis are regulated by feedback inhibition, in which the committed step is allosterically inhibited by the final product”. [6] b) Write short note on CAM pathway. [4] Q5) Attempt any two of the following. [10] a) Why is C4 pathway valuable for tropical plants. b) Which of the 20 amino acids can be synthesised directly from a common metabolic intermediate by a transamination reaction. c) Write short note on energy generation pathway in bacteria where sulfate acts as terminal electron aceptor. 