april 2022 sem 3 que papers

 MBCT 231 : Immunology

Q1) Solve any five of the following : [10] a) What are defensins? b) What is the significance of negative selection? c) What is the role of pathogen recognition receptors? d) What are knockout mice? e) Define lymphoma and give its types. f) What is Hodgkin’s disease? Q2) Attempt the following : a) Describe structure and function of B cell receptor. [7] b) Discuss the role of intracellular signalling proteins in immune activation mechanisms. [5] SEAT No. : P.T.O. [5834]-301 2 Q3) Attempt the following : a) What are transgenic animals? Give their significance in experimental immunology. [7] b) Describe Elispot assay. Give its applications. [5] Q4) Attempt the following : a) Explain IL-2 pathway - JAK/STAT of signal transduction. [7] b) Describe structure and function of TCR - CD3 complex. [5] Q5) Attempt the following : a) Discuss mechanisms of induction of immunological tolerance using transgenic animals. [7] b) Comment on T cell Mediated Supression of immune response. [5] Q6) Attempt the following : a) Explain immunosurveillance theory. [7] b) What are the methods used for diagnosis of tumours. [5] Q7) Write short notes on any two : [12] a) Cancer therapy. b) Tumour markers. c) Regulation of classical pathway of complement activation. 

MBCT - 232 : Molecular Biology (Rev. 2019)

Q1) Solve any five of the following : [10] a) What is trade - off mechanism? Give an example. b) Explain an example of SNP in humans. c) Enlist the techniques used in gene sequencing. d) Give 2 examples of amino acids with aromatic R groups. e) Give 2 applications of proteomics. f) Give 2 characteristics of Drosophila transposons. Q2) Attempt the following : a) Write the steps involved in separation and purification of proteins in a crude extract of microbial cells. [7] b) Write the characteristics of Tn5 and Tn10. [5] SEAT No. : P.T.O. [5834]-302 2 Q3) Attempt the following : a) Add a relative description of the replicative and non-replicative transposons. [7] b) “Histone modification is an example of epigenetic change” explain. [5] Q4) Attempt the following : a) How do telomeres play a central role in gene aging? [7] b) What is genetic trade-off mechanism? Write its significance. [5] Q5) Attempt the following : a) What are the important parts of gene casettes involved in integrons and also mention their roles? [7] b) What are the essential steps involved in the study of metabolomics? [5] Q6) Attempt the following : a) The short DNA shown below is to be sequenced, using your knowledge of how the Sanger method works. Dideoxynucleotides (dd NTPs) are added in relatively small amounts. Asteris R represents radioactive label. What length of strands will be synthesized on the 3 5    template below *5 ---3 OH   3 ---ACGACGCAGGACATTAGA3 5    Nucleotide mixtures added in below, dGTP, dATP, dTTP, dCTP, ddTTP [7] b) What is alternative gene expression? Give examples. [5] Q7) Write short notes on any two of the following : [12] a) Social and ethical concerns of genetically modified organisms. b) Regulation of E. coli. lac promoter. c) Methods used in Protein structure analysis.

MBCT - 233 : Clinical Microbiology

Q1) Attempt any five of the following : [10] a) Enlist steps in the mechanism of bacterial pathogenecity. b) Give characteristics of bacterial endotoxins with example. c) What are bacterial mechanisms to overcome host phagocytic defence? d) Enlist morphological forms in the life cycle of Ascaris lumbricoides. e) Enlist various proteins in HIV which have role in the pathogenesis. f) “Sulfur granules are pathogenic in nature” - Explain. Q2) Attempt the following : a) Describe morphological and cultural characteristics of Campylobacter jejuni. [7] b) Describe membrane ruffling in bacterial pathogenesis. [5] SEAT No. : P.T.O. [5834]-303 2 Q3) Attempt the following : a) Explain pathogenesis mechanism of hemorrhagic fever. [7] b) Compare and Contrast between ‘Susceptible Infectious Recovered’ model and ‘Susceptible Exposed Infectious Recovered’ model. [5] Q4) Attempt the following : a) Discuss in detail. Pathogenesis mechanism of Candida albicans infection along with steps involved. [7] b) Describe the mechanism of granuloma formation in latent phase of tuberculosis. [5] Q5) Attempt the following : a) Write a note on pathogenicity islands. Enlist their functions with suitable examples. [7] b) Suggest diagnostic methods for biological sample collected from patient suffering from peptic ulcer. [5] Q6) Attempt the following : a) Describe virulence factors in Acinetobacter baumanii. [7] b) Describe pathogenesis and clinical manifestations of amoebiasis. [5] Q7) Write short notes on any two : [12] a) Germ tube test for diagnosis of Candida albicans. b) Molecular techniques for detection of HIV antigens and viral nucleic acids. c) Structure of Hepatitis B virus.

MBET 236 : Bioremediation & Biomass Utilization

Q1) Attempt any five of the following : [5] a) Give any two examples of microbes used for bioremediation. b) Give any one disadvantage of bioremediation. c) Draw structure of Xylene. d) Enlist the components of lignocellulose. e) Draw structure of cellobiose. f) Enlist starch degrading enzymes. SEAT No. : P.T.O. [5834]-305 2 Q2) Attempt following : a) Write a note on types of cellulases with their mode of action. [6] b) What are the advantages of bioremediation? [4] Q3) Attempt following : a) How alterations in yeast transcription are useful for improvement of alcohol production? [6] b) Write steps in commercial production of sweetner used in the manufacturing of foods & beverages. [4] Q4) Attempt following : a) Explain how manipulations by plasmid transfer is useful for aromatic compound degradation. [6] b) Describe the isolation & manipulation of prokaryotic cellulase genes. [4] Q5) Add a short note on any two of the following : [10] a) Factors affecting bioremediation process. b) Commercial production of alcohol from cellulose. c) Camphor Degradation pathway.