2022 que paper for sem3

 MBCT 231 : Immunology

Q1) Solve any five of the following. [10] 

a) What are Toll like receptors? 

b) What is sthe role of PAMPs in innate immune response.

 c) What is SCID mice? 

d) What is negative selection? 

e) Write principle of Elispot assay. 

f) What is Hodgkin’s disease? 

Q2) Attempt the following. 

a) Describe IL-2 pathway of signal tansduction. [7]

 b) Justify-TCR-CD3 complex is required for activation mechanism. [5] 

Q3) Attempt the following. 

a) Explain Network theory of antigen antibody complex formation. [7] 

b) How biological response modifiers can be used for cancer therapy. [5] 

Q4) Attempt the following. 

a) What are transgenic animals? How they are used in immunological research? [7] 

b) Explain the methods used for funcitonal assay of macrophages. [5] 

Q5) Attempt the following. 

a) Comment on the escape mechanisms of tumour from host defense system. [7] 

b) Describe different types of tumours of lymphoid system. [5] 

Q6) Attempt the following. 

a) How animal models are used in the study of atoimmunity and AIDS. [7]

 b) Explain cytokine mediated cross regulation of T cells. [5] 

Q7) Write short notes on any two. [12] 

a) RAS/MAP kinase pathway. 

b) Regulation of classical pathway of complement activation. 

c) Structure and functions of B cell receptor. 

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MBCT-232 : MOLECULAR BIOLOGY

Q1) Solve any five of the following [10] a) Name any two techniques used for protein characterization. b) Mention any two applications of transgenic animals. c) Give two examples of amino acids with non-polar, aliphatic ‘R’ groups. d) Give two examples of eukaryotic expression vectors. e) What is gene-aging? Give one example. f) What is SNP? Give one example. Q2) Attempt the following: a) “ Telomere length can be an indicate of an individual’s biological age” justify. [7] b) What is longevity? Mention the biological factors effecting tongevity.[5] Q3) Attempt the following. a) The first five nucleotide pairs of a particular gene are present in following sequence, A T G C A T A C G T ↑ How many distinct homo alleles of the above gene are there that differ from the above sequence by a single nucleotide substitution occuring at position 5 (nucleotide pair No. 5 as indicated by arrow.) [7] b) “ Humans can be an example of alternative gene expression” explain.[5] P.T.O. 2 [5913]-32 Q4) Attempt the following: a) What is the relation between genetic trade off and evolution? [7] b) “ Small non-coding RNA regulate the epigenetics” explain. [5] Q5) Attempt the following. a) Sometimes the strategy for the expression of a target protein in a host organism involves synthesizing the protein as a part of fusion protein. What are the advantages of this approach? [7] b) Give two examples of each. i) Amino acids with positively charged R group. ii) Amno acids with negatively charged R group. [5] Q6) Attempt the following. a) Which of the following pairs of DNA sequences could qualify as the terminal repeats. of a bacterial IS element? Explain why. i) 5'–GAATCCGCA'–3' and 5'–ACGCCTAAG'–3' ii) 5'–GAATCCGCA–3' and 5'–CTTAGGCGT–3' iii) 5'–GAATCCGCA–3' and 5'–TGCGGATTC–3' [7] b) What are the differences between replicative and non-replicative transposition? [5] Q7) Write short notes on any two of the following. [12] a) Composite transposons. b) Advantages and disadvantages of geritically modified organisms. c) What is gene-argumentation? Add a note on its significance. 

MBCT-233 : Clinical Microbiology

Q1) Attempt any five of the following. [10] a) What is biofilm formation? b) What are exotoxins? Give two examples. c) Write two characters of antigenically variable component of endotoxin. d) Enlist the morphological forms of Giardia lamblia. e) Enlist various methods for HIV detection. f) Draw neat labelled diagram of ahon’s complex. Q2) Attempt the following. a) Describe in detail the disease prediction model of covid 19. [7] b) Describe immunoprecipitation and enzyme immunoassay techniques used in in-vitro detection of bacterial toxins. [5] Q3) Attempt the following. a) Describe antimicrobial resistance mechanisms developed by Acinetobacter baumanii. [7] b) Describe characteristic features and cultural characteristics of etiological agent of tuberculosis. [5] P.T.O. 2 [5913]-33 Q4) Attempt the following. a) Discuss in detail adherence and colonization mechanism in bacterial pathogenesis. [7] b) Describe clinical manifestations of infection with dermatophyte fungus Trichophyton Mentagrophytes. [5] Q5) Attempt the following. a) Write a note on treatment used for HIV-AIDS. Describe preventive measures for it. [7] b) Suggest treatment and precautionary measures for H1N1 swine flu. [5] Q6) Attempt the following. a) Describe morphology and detailed life cycle of Ascaris lumbricoides. [7] b) Describe pathophysiology of Ebola virus infection. [5] Q7) Write short notes on any two. [12] a) Hair perforation test for dermatophytic fungi. b) Rapid antigen test and RT - PCR test for detection of Influenza A. c) Structure of HIV particle. 

MBET 236 : Bioremediation & Biomass Utilization

Q1) Attempt any five of the following : [5] a) Give any two examples of plasmids used for creation of super bug. b) What do you mean by in-situ bioremediation. c) Draw structure of napthalene. d) Give two examples of cellulose degrading micro organisms. e) What is the advantage of using biomass as fuel source? f) Give two examples of micro organisms used for industrial alcohol production. Q2) Attempt following : a) Explain the isolation and manipulation of eukaryotic cellulose genes. [6] b) Explain the important methods involved in microbial degration of Xenobiotics. [4] P.T.O. [5913]-35 2 Q3) Attempt following : a) Explain genetic engineering of 2 mobilis for utilization of different carbon sources for production of ethanol. [6] b) Describe silage production & it’s advantages. [4] Q4) Attempt following : a) Explain modulation of yeast transcription for improvization of alcohol production. [6] b) Explain Napthalene biodegradation pathway. [4] Q5) Add a short note on any two of the following : [10] a) Advantages of bioremediation. b) Degradation of lignin. c) Development of Xenobiotic degrading bacteria by using suitable example. 