MSC Guruji

 MB - 601 : Instrumentation & Molecular Biophysics Q1) Attempt any two of the following. [10] a) Explain significance of van deemter equation in chromatography. b) Explain any two applications of affinity chromatography. c) A macromolecule with V partial specific volume is 0.74 cm3/g is redimented in water at 20ºC S is 14.210–13 , D is 5.8210–6 , R is 8.3100107 calculate its mole cular weight. Q2) Attempt any two of the following. [10] a) Enlist different methods of ionization in Mass Spectroscopy and explain any one method in detail. b) Diagrammatically represent circular dichroism Instrument and Explain any 2 applications of C.D. c) Write short note on : FRET (Fluorescence Resonance Energy Transfer) Q3) Attempt any two of the following. [10] a) Explain Direct Lattice and Reciprocal Lattice seen in x-ray crystallography b) Explain Chemical Shift and Intensity with respect to NMR c) Comment on : NOESY P.T.O. 2 [5534]-201 Q4) Attempt any two of the following. [10] a) Explain ...

 Credit 1: Genomics Credit 2: Genertically modified plants and animals Credit 3: Mobile DNA elements Credit 4: Proteomics IA Attempt any two of the following : [10] a) Describe the three hybrid assay technique in yeast genetics. b) Comment on - DMS foot printing as tool in molecular biology. c) State the significance of RFLP and its association with disease prediction. PA Attempt any two of the following : a) Explain the mechanism of negative control of lac operon. b) Comment on : repression loop in the fine control of ara operon. c) Justify - Attenuation is required in regulation of trp operon. €F„FyF [10] QA Attempt any two of the following : [10] a) Diagrammatically illustrate formation of mature mRNA from its pre- mRNA transcript. b) What is RNA interference in r RNA processing? c) Comment on : Si RNA. RA Attempt any two of the following : [10] a) Give a protocol for preparation of a DNA probe. b) Explain the singificance of sigma factor switching in phage infection. c) Com...

Credit 1: Cell surface molecules and receptors 2 mark a) What are Toll like receptors? a) Describe the role of Toll like receptors in innate immunity.  c) What is the role of pathogen recognition receptors?  a) What are defensins?  a) What are primary cell cultures and cell lines?  b) Explain in brief cytokine receptor families, giving suitable examples. c) With the help of diagram, explain structure of B cell receptor. a) Explain the general properties of cytokines giving examples. b) Explain the T cell accessory membrane molecules and its role in immune activation. 5/7 mark c) Regulation of classical pathway of complement activation.  b) Diagrammatically explain regulation of classical complement pathway.   b) Regulation of classical pathway of complement activation. c) Explain the role of TCR - CD3 complex in activation of immune response. c) Explain the role of Antigen and Antigen - Antibody complexes in the regulation of immune response. a) Descri...

 immunology  Q1) Attempt any two of the following : [10] a) Explain the structure of B-cell receptor. b) Describe the role of adhesion molecules in immune activation. c) Describe JAK / STAT signal transduction pathway. Q2) Attempt any two of the following : [10] a) Explain the mechanisms of tolerance induction by giving experimental evidences. b) Diagrammatically explain regulation of classical complement pathway. c) Explain the role of Antigen in regulation of immune response. Q3) Attempt any two of the following : [10] a) What are primary cell cultures and cell lines? b) Describe ELISPOT assay and give its application. c) Describe the use of animal models in studying AIDS. P.T.O. 2 [5534]-301 Q4) Attempt any two of the following : [10] a) Describe the role of Toll like receptors in innate immunity. b) Describe Idiotypic network theory and its experimental evidence. c) Describe functional assays for phagocytes. Q5) Attempt any two of the following : [10] a) Describe escape me...

 MB - 601 : Instrumentation & Molecular Biophysics Q1) Attempt any two of the following. [10] a) Explain significance of van deemter equation in chromatography. b) Explain any two applications of affinity chromatography. c) A macromolecule with V partial specific volume is 0.74 cm3/g is redimented in water at 20ºC S is 14.210–13 , D is 5.8210–6 , R is 8.3100107 calculate its mole cular weight. Q2) Attempt any two of the following. [10] a) Enlist different methods of ionization in Mass Spectroscopy and explain any one method in detail. b) Diagrammatically represent circular dichroism Instrument and Explain any 2 applications of C.D. c) Write short note on : FRET (Fluorescence Resonance Energy Transfer) Q3) Attempt any two of the following. [10] a) Explain Direct Lattice and Reciprocal Lattice seen in x-ray crystallography b) Explain Chemical Shift and Intensity with respect to NMR c) Comment on : NOESY P.T.O. 2 [5534]-201 Q4) Attempt any two of the following. [10] a) Explain ...

 MBCT 231 : Immunology Q1) Solve any five of the following : [10] a) What are defensins? b) What is the significance of negative selection? c) What is the role of pathogen recognition receptors? d) What are knockout mice? e) Define lymphoma and give its types. f) What is Hodgkin’s disease? Q2) Attempt the following : a) Describe structure and function of B cell receptor. [7] b) Discuss the role of intracellular signalling proteins in immune activation mechanisms. [5] SEAT No. : P.T.O. [5834]-301 2 Q3) Attempt the following : a) What are transgenic animals? Give their significance in experimental immunology. [7] b) Describe Elispot assay. Give its applications. [5] Q4) Attempt the following : a) Explain IL-2 pathway - JAK/STAT of signal transduction. [7] b) Describe structure and function of TCR - CD3 complex. [5] Q5) Attempt the following : a) Discuss mechanisms of induction of immunological tolerance using transgenic animals. [7] b) Comment on T cell Mediated Supression of immune ...

 MB - 802 : Molecular Biology - II Q1) Attempt any two of the following: [10] a) Explain the principle of pyrosefuencing method. b) Elaborate gene imprinting with suitable example. c) Explain the role of geromic variation in trade offs. Q2) Attempt any two of the following: [10] a) Give structural details of YAC and Enlist uses of YAC in cloning. b) Enlist methods for gene transfer to host cell. Explain any one in detail. c) Give the protocol of preparing genomic library. Q3) Attempt any two of the following: [10] a) How will you prepare ‘gum’ using RDT. b) How will you produce novel antibiotics using RDT. c) Explain the production of tryptophan using genetically modified microbes. Q4) Attempt any two of the following: [10] a) What is the correlation between SNP and certain diseases? b) Explain protein engineering with suitable example. c) Write a note on importance of production of high quality protein drug by unconventional microbial system. P.T.O. [5834] - 11 2 Q5) Attempt any t...

 MBCT 231 : Immunology Q1) Solve any five of the following. [10]  a) What are Toll like receptors?  b) What is sthe role of PAMPs in innate immune response.  c) What is SCID mice?  d) What is negative selection?  e) Write principle of Elispot assay.  f) What is Hodgkin’s disease?  Q2) Attempt the following.  a) Describe IL-2 pathway of signal tansduction. [7]  b) Justify-TCR-CD3 complex is required for activation mechanism. [5]  Q3) Attempt the following.  a) Explain Network theory of antigen antibody complex formation. [7]  b) How biological response modifiers can be used for cancer therapy. [5]  Q4) Attempt the following.  a) What are transgenic animals? How they are used in immunological research? [7]  b) Explain the methods used for funcitonal assay of macrophages. [5]  Q5) Attempt the following.  a) Comment on the escape mechanisms of tumour from host defense system. [7]  b) Describe diff...